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Genetic study of deletions and mutations in sperm mitochondrial 15.0 DNA in idiopathic asthenozoospermia, oligoasthenoteratozoospermia of infertile men

Funding Agency: Indian Council of Medical Research, New Delhi
Duration of Study: July 2014- July 2016
Principal Investigator: Dr Asoke K Pal, Professor of Cytogenetics
Co-Investigator: Dr AR Chaudhari, Professor of Physiology
Ethics Approval: Obtained from Institutional Ethics Committee, MGIMS ( MGIMS/IEC/ ANAT/01/2014 dated 25.01.2014)

Married couples run a one in ten risk of being infertile and often are unable to cope with emotional, family and social problems infertility is associated with. Although infertility is a multi-factorial disorder, genetic etiologies play an important role. Semen analysis can be used as an index of human fertility through sperm concentration, motility, morphology, and quality. So, we designed this study to find out the relationship between sperm mitochondrial DNA deletions or mutations in astheno-zoospermia and oligo-astheno-terato-zoospermia and infertility.

The department of Physiology at MGIMS runs an semen analysis unit. We are recruiting 200 astheno-zoospermic and oligo-astheno-terato-zoospermic individuals and 50 fertile men aged between 22 and 45 years of age.

We extract the sperm DNA from the semen samples collected from infertile and fertile men. We amplify and sequence mtDNA genes and find out sperm mitochondrial DNA deletions or mutations by long range polymerase chain reaction (PCR), primer shift PCR method, PCR RFLP method, and semi quantitative PCR method.

Beginning July 2014, we have procured the instrument and chemicals and have standardized the techniques. A third of the target sample has been enrolled and after enrolling 200 individuals, we shall statistically analyse the results.

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Genetic study of deletion and mutation in sperm mitochondrial DNA in idiopathic astheno- zoospermia and oligoasthenoteratozoospermia

Funding Agency: Indian Council of Medical Research, New Delhi
Duration of Study:
Principal Investigator: Dr Asoke K Pal, Professor of Cytogenetics
Amount: 16 Lakhs
Ethics Approval:

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Evaluation of immunomodulatory effect and therapeutical potential of filarial proteins in experimental ulcerative colitis – in collaboration with Biochemistry Dept. MGIMS.

Funding Agency: DST
Duration of Study:
Principal Investigator: Dr. MVR Reddy, Dr MR Shende, Dr AM Tarnekar
Amount: 38.78 Lakhs
Ethics Approval:

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